Analysis Note

ControlHeLa cell lysate

Application

Use Anti-SRp55 Antibody, clone 9-1-56 (Mouse Monoclonal Antibody) validated in WB, IP, ICC, IHC to detect SRp55 also known as arginine/serine-rich splicing factor 6, Pre-mRNA-splicing factor SRP55.

Research Sub CategoryRNA Metabolism & Binding Proteins

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pre-mRNA-splicing factor SRp55 is a member of the serine/arginine-rich (SR) protein family. This group of proteins is found mainly in the nucleus where they play a critical role in regulated and constitutive splicing of precursor mRNAs. SRp55 is involved in the constitutive process for mRNA splicing, and is able to mediate alternative splice site selection. Considered one of the more ubiquitous of the splice factors, SRp55 is upregulated in response to DNA damage when p53 is lacking. SRp55 is significantly phosphorylated on RS domain serine residues.

Immunogen

MBP-tagged recombinant protein corresponding to human SRp55.

Epitope: Not determined

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without 0.05% sodium azide.

Protein G

Format: Purified

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SRp55 on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 43 kDa observed. 40 kDa calculated.